agei ecori (New England Biolabs)
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New England Biolabs
agei ecori
Agei Ecori, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 15540 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ecori/EcoRI/pmc13693670-239-9-10
Average 99 stars, based on 15540 article reviews
Agei Ecori, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 99/100, based on 15540 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/ecori/EcoRI/pmc13693670-239-9-10
Average 99 stars, based on 15540 article reviews
agei ecori - by Bioz Stars,
2026-11
99/100 stars
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Amplification:Article Title: In vivo DNA damage protection during cell migration across confining embryonic tissue environments. Article Snippet: mApple-53BP1 and BAF-mCherry were subcloned into a pCS2+ vector for mRNA synthesis from Addgene plasmids 69531 ref. 58 and 172447 (ref. 100). .. For mApple-53BP1, pCS2+ was digested with BamHI and Article Title: Results Of PMP22 Gene Deletion and Duplication Analysis in 100 Patients with Hereditary Neuropathy Article Snippet: .. The amplified products were digested with 10 U Purification:Article Title: Detergent-based preparation influences TMEM16F assembly and scramblase activity. Article Snippet: The amplified fragment was separated by electrophoresis on a 0.8% agarose gel and purified using the FastGene Gel/PCR Extraction Kit (NIPPON Genetics, Tokyo, Japan). .. Both the purified PCR product and the p3xFLAG-CMV-14 vector were digested with Polymerase Chain Reaction:Article Title: Detergent-based preparation influences TMEM16F assembly and scramblase activity. Article Snippet: The amplified fragment was separated by electrophoresis on a 0.8% agarose gel and purified using the FastGene Gel/PCR Extraction Kit (NIPPON Genetics, Tokyo, Japan). .. Both the purified PCR product and the p3xFLAG-CMV-14 vector were digested with Plasmid Preparation:Article Title: Detergent-based preparation influences TMEM16F assembly and scramblase activity. Article Snippet: The amplified fragment was separated by electrophoresis on a 0.8% agarose gel and purified using the FastGene Gel/PCR Extraction Kit (NIPPON Genetics, Tokyo, Japan). .. Both the purified PCR product and the p3xFLAG-CMV-14 vector were digested with Article Title: Mechanical characterization of human centromeres Article Snippet: .. These two DNA fragments were designed to have a 25 nt overlap with adjoining fragments and were assembled into the pUC19 vector digested with Article Title: MSMEI_4050 modulates resistance to the antitubercular drug rifampicin in Mycolicibacterium smegmatis. Article Snippet: .. The MSMEI_4050 gRNA in the PLJR962 vector was cloned using gBlocksTM (IDT, IA, USA) with the gRNA sequence inside along with the vector sequence to obtain a 183 pb final sequence, which was subsequently cloned and inserted into a PLJR962 vector using Article Title: MaltEms-MaltOBP9 axis mediates sexually dimorphic olfactory behavior in Monochamus alternatus via recognition of pheromone 2-methoxy-4-vinylphenol. Article Snippet: The olfactory system is essential for insect survival and behavior, with odorant-binding proteins (OBPs) playing a key role by binding and transporting chemical signals to olfactory receptors.. Although MaltOBP9 from Monochamus alternatus, a primary vector of pine wilt disease, has been linked to olfactory functions in M. alternatus, its regulatory mechanisms and specific roles are not well understood.. In this study, we show that MaltOBP9 is highly expressed in the antennae of both male and female adults after sexual maturation. Ligation:Article Title: Detergent-based preparation influences TMEM16F assembly and scramblase activity. Article Snippet: The amplified fragment was separated by electrophoresis on a 0.8% agarose gel and purified using the FastGene Gel/PCR Extraction Kit (NIPPON Genetics, Tokyo, Japan). .. Both the purified PCR product and the p3xFLAG-CMV-14 vector were digested with Agarose Gel Electrophoresis:Article Title: Biocatalytic synthesis of S-adenosyl-l-methionine analogues for allosteric activation of cystathionine-β-synthase. Article Snippet: Reactions of 25 μL containing 0.5 μg Lambda DNA (N3013S, New England Biolabs), 20U EcoRI methyltransferase (M0211S, New England Biolabs), 10× Methyltransferase buffer (M0211S, New England Biolabs) and 10 μM commercial SAM (B9003S, New England Biolabs) or SAM analogue (10 μM, as per phosphate) were incubated at 37 °C for 1 h, after which EcoRI methyltransferase was inactivated by heating to 65 °C for 20min. .. Unmethylated DNA was digested with 10U Article Title: Results Of PMP22 Gene Deletion and Duplication Analysis in 100 Patients with Hereditary Neuropathy Article Snippet: .. The amplified products were digested with 10 U Derivative Assay:Article Title: Visual and Equipment-Minimized Detection of Avian Metapneumovirus Subtype C by RT-RPA-CRISPR/Cas12a Coupled with Lateral Flow Dipstick. Article Snippet: .. The LbCas12a endonuclease (derived from Lachnospiraceae bacterium), alongside Clone Assay:Article Title: MSMEI_4050 modulates resistance to the antitubercular drug rifampicin in Mycolicibacterium smegmatis. Article Snippet: .. The MSMEI_4050 gRNA in the PLJR962 vector was cloned using gBlocksTM (IDT, IA, USA) with the gRNA sequence inside along with the vector sequence to obtain a 183 pb final sequence, which was subsequently cloned and inserted into a PLJR962 vector using Sequencing:Article Title: MSMEI_4050 modulates resistance to the antitubercular drug rifampicin in Mycolicibacterium smegmatis. Article Snippet: .. The MSMEI_4050 gRNA in the PLJR962 vector was cloned using gBlocksTM (IDT, IA, USA) with the gRNA sequence inside along with the vector sequence to obtain a 183 pb final sequence, which was subsequently cloned and inserted into a PLJR962 vector using Expressing:Article Title: MaltEms-MaltOBP9 axis mediates sexually dimorphic olfactory behavior in Monochamus alternatus via recognition of pheromone 2-methoxy-4-vinylphenol. Article Snippet: The olfactory system is essential for insect survival and behavior, with odorant-binding proteins (OBPs) playing a key role by binding and transporting chemical signals to olfactory receptors.. Although MaltOBP9 from Monochamus alternatus, a primary vector of pine wilt disease, has been linked to olfactory functions in M. alternatus, its regulatory mechanisms and specific roles are not well understood.. In this study, we show that MaltOBP9 is highly expressed in the antennae of both male and female adults after sexual maturation. |